Coding
Part:BBa_K2121012:Design
Designed by: Leslie Wang Group: iGEM16_Yale (2016-10-21)
Rhamnose_Lambda_Beta_F7X3D1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 587
Illegal NgoMIV site found at 965 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Expression fusion with native S. meliloti protein.
Source
Promoter sequence from S. meliloti genome. Recombinase sequence identified from homology to the lambda coliphage beta recombinase via BLASTp search.